As artificial intelligence transforms economies and societies worldwide, preparedness of higher education institutions has become essential for sustainable development and competitiveness. This comprehensive checklist provides a strategic framework for HEIs in Oman to assess and enhance its AI readiness across critical dimensions: strategic governance, digital infrastructure, workforce development, research and innovation, economic integration, ethical frameworks. Aligned with Oman Vision 2040, this resource guides policymakers, institutions, and stakeholders in building a robust, inclusive, and forward-thinking AI ecosystem that positions the Sultanate as a regional leader in responsible AI adoption and innovation.